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بررسی اثرات آنتیاکسیدانی فیکوسیانین بر سلولهای سرطان کولون در in vitro و in vivo | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 10، شماره 2 - شماره پیاپی 38، آبان 1400، صفحه 21-30 اصل مقاله (448.86 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2021.58018.1821 | ||
| نویسندگان | ||
| لیلا نجفی* 1؛ محمد فضیلتی2؛ حسین صلواتی3 | ||
| 1دانشجوی دکتری بیوشیمی، دانشکده علوم، دانشگاه پیام نور اصفهان، ایران | ||
| 2استاد بیوشیمی، دانشکده علوم، دانشگاه پیام نور اصفهان، ایران | ||
| 3دانشیار، گروه شیمی، دانشکده علوم، دانشگاه پیام نور اصفهان، ایران | ||
| چکیده | ||
| فیکوسیانین دارای خصوصیات درمانی بدون اثر سمیتی میباشد. هدف این مطالعه بررسی اثر آنتیاکسیدانی و ضد سرطانی فیکوسیانین روی سلولهای CT-26 و HT-29 و در موشهای Balb/c میباشد. این سلولها با غلظتهای µg/ml100-1 فیکوسیانین بهمدت 48 ساعت تیمار شدند و اثرات ضد تکثیری آن با روشهای مورفولوژیکی، رنگآمیزی AO/PI و DAPI، تست MTT، میکروسکوپ فلورسانس و فلوسایتومتری بررسی شد. در فاز حیوانی موشها سرطانی شده و به چهار گروه 1- کنترل، 2- تیمار با فیکوسیانین mg/kg50، 3- تیمار با سیلیمارین mg/kg100 و 4- سیسپلاتین mg/kg 3 تقسیم شدند. بعد از گذشت 4 هفته سطوح سرمی MDA، TAC، بیلیروبین، پروتئین تام، آلبومین و فعالیت آنزیمهای GPX، CAT، SOX، AST، ALT، LDH سنجش شدند. فیکوسیانین اثرات ضد تکثیری قابلتوجه با میزان µg/ml4/47=50IC برای رده CT-26 و µg/ml4/49=50 IC برای رده HT-29 نشان داد. نتایج نشان داد که فیکوسیانین بهطور معنادار 001/0P<، میزان MDA را کاهش و میزان آنزیمهای آنتیاکسیدانی را افزایش داد. | ||
| کلیدواژهها | ||
| آنتیاکسیدان؛ فیکوسیانین؛ موش | ||
| عنوان مقاله [English] | ||
| Antioxidant Effects of C-Phycocyanin in Colon Cancer Cell Lines invitro and invivo | ||
| نویسندگان [English] | ||
| Leyla Najafi1؛ Mohammad Fazilati2؛ Hossein Salavati3 | ||
| 1Ph.D., Biochemistry Department, Payame Noor University, Iran | ||
| 2Professor of Biochemistry Department, Payame Noor University, Iran | ||
| 3Associate Professor of Chemistry Department, Payame Noor University, Iran | ||
| چکیده [English] | ||
| C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various concentrations of C-PC extract (1-100µg/ml) in 48hr. Antiproliferative effect was measured by morphological observations, DAPI and AO/PI Staining, MTT assay, fluorescence microscope and Flow cytometry assays. Antioxidant effects of C-PC on CT-26 tumor cells transplanted in Balb/c mice was also checked out invivo. Male Balb/c mice were tested in four groups.group1 was considered as control. Group 2 were fed by C-Phycocyanin (50mg/kg) daily. In groups 2-4, cisplatin (3mg/kg) was injected, and group 3 silymarin (100mg/kg) was injected daily. Finally serum levels of MDA, TAC, and Total Billirubin, Total Protein and Albumin and activities of GPX, Catalase, SOD, ALT, AST, and LDH were assayed. C-PC showed considerable anti-proliferative effect on CT-26 and HT-29 tumor cell lines with IC50 =47.4 µg/ml and IC50=49.4 µg/ml respectively. In addition, C-PC because of its antioxidant potential, significantly (P<0/001), decreased MDA and increased levels of liver antioxidant enzymes. | ||
| کلیدواژهها [English] | ||
| Antioxidant, Mice, Phycocyanin | ||
| مراجع | ||
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