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تاثیر سولفورافان موجود در عصاره گیاه Brassica oleracea بر دو فاکتور سیستم ایمنی TNF و IL-6، در اختلالات طیف اتیسم | ||
فصلنامه علمی زیست فناوری گیاهان زراعی | ||
دوره 10، شماره 3 - شماره پیاپی 35، آذر 1400، صفحه 79-98 اصل مقاله (856.74 K) | ||
نوع مقاله: علمی پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/cb.2021.57154.1829 | ||
نویسندگان | ||
مهدیه شجاعی1؛ راضیه سرآبادانی* 2 | ||
1کارشناسارشد، گروه بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران. | ||
2استادیار، گروه بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران. | ||
چکیده | ||
اختلالات سیستم ایمنی یکی از دلایل ابتلای افراد به بیماری اتیسم است. TNF و IL-6 از نشانگرهای مهم بیماری اتیسم میباشند. افزایش بیش از اندازه این سایتوکاینها در مغز و خون بیماران اتیسمی علاوه بر اختلالات سیستم ایمنی، زمینهساز بسیاری از ناهنجاریهای رفتاری شایع در مبتلایان به این بیماری میباشد. در این پژوهش، سللاین عصبی 1321N1 و سللاین HFF بهعنوان سلولهای نرمال تحت القای سیستم ایمنی قرار گرفتند تا فاکتورهای ایمنی در بالاترین سطح از فعالیت خود قرار گیرد، سپس سلولها با سه غلظت 1، 5/1 و 2 میلیگرم بر میلیلیتر از عصاره کلمبروکلی تیمار شدند و در سه بازه زمانی 1، 2 و 3 روزه توسط تست الایزا مورد آنالیز قرار گرفتند و اثر سولفورافان موجود در عصاره کلمبروکلی بر روی دو فاکتور TNF و IL-6 بررسی شد. نتایج مقایسه نمونه بدون تیمار با عصاره کلمبروکلی و نمونه تیمار شده با 2 میلیگرم از عصاره کلمبروکلی بهمدت 3 روز، نشان داد که دو فاکتور TNFαو IL-6 تحتتاثیر عصاره کلمبروکلی کاهش یافت. همچنین مشاهده شد که غلظت بالاتر عصاره و زمان بیشتر تیمار دهی اثر بخشی بیشتری در کاهش دو فاکتور مذکور داشت. بهطور کلی نتایج آزمایش حاضر تاثیر عصاره کلمبروکلی بر کاهش التهاب سیستم ایمنی که بهواسطه این دو فاکتور ایجاد شدند، را پیشنهاد میدهد. | ||
کلیدواژهها | ||
عصاره کلم بروکلی؛ سیستم ایمنی؛ IL6؛ TNF&alpha | ||
عنوان مقاله [English] | ||
Effect of Brassica oleracea Extract on TNFα and IL-6 as Two Factors of Immune System in Relation to Autism Spectrum Disorder | ||
نویسندگان [English] | ||
Mahdieh Shojaee1؛ Razieh Sarabadani2 | ||
1M.Sc., Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran. | ||
2Assistant Professor, Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran. | ||
چکیده [English] | ||
Immune system disorders have been recognized as a cause of the autism spectrum disorder (ASD). TNFα and IL-6 are significant markers of the ASD. Increased levels of these cytokines in the brain and blood of the ASD-engaged patients not only affect the immune system disorders but also contribute to many common behavioral abnormalities exhibited by such patients. In this research, we investigated the effect of the broccoli extract and its sulforaphane content on two immune system factors, namely TNFα and IL-6. For this purpose, with its effectiveness confirmed via gas chromatography – mass spectroscopy (GC-MS) and MTT assay, the broccoli extract was subjected to immune system induction to maximize the activity of the immune system factors. The cells were treated with the broccoli extract at three different dosages, namely 1, 1.5 and 2 mg of the cell per milliliter of the broccoli extract, enzyme-linked immunosorbent assays (ELISA) were performed for three treatment times, namely 1, 2, and 3 days. A comparison between the stimulated immune system cells without treatment against those treated with the 2 mg of broccoli extract for 3 days clearly demonstrated the effectiveness of the broccoli extract for attenuating the activity of the TNFα and IL-6 factors. It was also observed that higher concentrations of the extract and longer treatment time were more effective in reducing these two factors. In general, the results of the present experiment suggest the effect of broccoli extract on reducing the inflammation of the immune system caused by these two factors. | ||
کلیدواژهها [English] | ||
broccoli extract, immune system, IL-6, TNF&alpha | ||
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