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مطالعه گسترده خانواده ژنی پروتئینهای مسئول مقاومت به فلزات (MTP) در یونجه (Medicago truncatula) ، لوبیا(Phaseolus vulgaris) و سویا (Glycine max) | ||
فصلنامه علمی زیست فناوری گیاهان زراعی | ||
دوره 10، شماره 3 - شماره پیاپی 35، آذر 1400، صفحه 63-77 اصل مقاله (1.03 M) | ||
نوع مقاله: علمی پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/cb.2022.60877.1854 | ||
نویسندگان | ||
زهرا پاکباز1؛ آسا ابراهیمی* 2؛ مارتینا ریکاور3؛ سیسیل بن3؛ عبدالله محمدی4 | ||
1گروه بیوتکنولوژی و به نژادی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
2گروه بیوتکنولوژی و به نژادی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران. | ||
3آزمایشگاه اکولوژی و محیط زیست کاربردی، دانشگاه تولوز، CNRS، تولوز، فرانسه. | ||
4دانشیار، گروه اصلاح نباتات، واحد کرج، دانشگاه آزاد اسلامی، تهران، ایران. | ||
چکیده | ||
پروتئینهای مسئول تحمل به فلزات (MTP) از جمله ناقلهای کاتیونی دو ظرفیتی در دیواره سلولی گیاهان هستند که نقش مهمی را در رشد گیاهان ایفا میکنند. آنها در فرایند جذب فلزات ریزمغذی و ایجاد مقاومت در گیاهان در خاکهای آلوده به فلزات سنگین شرکت میکنند. با این حال اطلاعات کافی در مورد ژنهای MTP در خانواده گیاهی بقولات بهاندازه کافی وجود ندارد. بنابراین ما در این مطالعه، ارزیابی گستردهای از ژنهای MTP در سه عضو مهم این خانواده شامل: یونجه (Medicago truncatula)، لوبیا (Phaseolus vulgaris) و سویا (Glycine max) با بررسی روابط فیلوژنتیکی، نحوه توزیع کروموزومی، ساختار ژنی و بیان آنها در بافتهای مختلف فراهم آوردیم. با توجه به نتایج بهدست آمده 14، 12 و 23 عدد ژن MTP بهترتیب در یونجه، لوبیا و سویا یافت شد. 13 ژن MTP مضاعف در سویا یافت شد در حالی که در لوبیا و یونجه هیج مضاعفشدگی یافت نشد. همهMTP های موردمطالعه در هر سه گیاه در سه گروه Mn-CDFs، Zn-CDFs و Fe/Zn-CDFs دستهبندی شدند. نتایج بررسی جایگاه زیر سلولی به روش In silico نشان داد که بیشترین فعالیت این پروتئینها در هر سه گیاه در واکوئل میباشد و تعداد کمی در دیواره سلولی و هسته قرار دارند. بررسی ساختار ژنی و پروتئینی این ژنها در این گیاهان حاکی از حفاظتشدگی بالای این پروتئینها بود اما هرکدام از آنها سطوح مختلفی از بیان ژن را در طی رشد نشان دادند. این امر میتواند حاکی از نقش مهم این پروتئینها در طی رشد و نمو گیاهان باشد. | ||
کلیدواژهها | ||
بقولات؛ بیوانفورماتیک؛ خانواده ژنی؛ فلزات سنگین | ||
موضوعات | ||
بیوانفورماتیک | ||
عنوان مقاله [English] | ||
Genome‑wide study of metal tolerance protein (MTP) family in Medicago truncatula , Phaseolus vulgaris and Glycine max . | ||
نویسندگان [English] | ||
Zahra Pakbaz1؛ Asa Ebrahimi2؛ Martina Rickauer3؛ Cecile Ben3؛ Abdollah Mohammadi4 | ||
1Department of Plant Breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran. | ||
2Department of Plant Breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran. | ||
3Laboratoire Ecologie Fonctionnelle et Environnement, Université de Toulouse, CNRS, Toulouse, France. | ||
4Associate Professor, Department of Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran. | ||
چکیده [English] | ||
Metal tolerance proteins (MTP) are plant membrane divalent cation transporters, which plays an important role during plant growth and development. They involve in minerals uptake and provide resistance for plants in polluted soil by heavy metal. However, information about MTPs proteins in Fabceace family are scarcely known. Therefore, in this study we provided an extensive evaluation of MTP genes in three important members of this family including: Glycine max, Medicago truncatula and Phaseolus vulgaris by providing phylogenetic assessments, chromosomal distributions, gene structures and expression in different tissue. According to the results 14, 12 and 23 MTP genes respectively were found in M. truncatula, P. vulgaris and G. max. 13 duplicated MTP genes in G. max were found meanwhile we did not find any duplication in the MTP genes of M. truncatula and P. vulgaris. All studied MTPs were classified into three major cation diffusion facilitator (CDFs) groups; Mn-CDFs, Zn-CDFs, and Fe/Zn-CDFs. In silico subcellular location results revealed that these proteins have the maximum activity in the vacuole in all three plants, and a small number are located in the cell wall and nucleus. According to gene structure and protein motifs of studied MTPs, they are highly conserved but their expression measurement showed that each one of them have different levels of expression during growth stage. It confirms their importance for plants during growth and development. | ||
کلیدواژهها [English] | ||
Fabceace, Heavy metal, Bioinformatics, Gene family | ||
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