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بررسی بیان ژنهای کاتالاز و DREB-2 تحت تنش کمآبی در ژنوتیپهای ذرت | ||
فصلنامه علمی زیست فناوری گیاهان زراعی | ||
دوره 10، شماره 4 - شماره پیاپی 36، اسفند 1400، صفحه 75-93 اصل مقاله (608.63 K) | ||
نوع مقاله: علمی پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/cb.2022.62784.1869 | ||
نویسندگان | ||
خسرو مفاخری* 1؛ مصطفی ولی زاده2؛ سیدابوالقاسم محمدی3 | ||
1دانشآموخته دکتری اصلاح نباتات (ژنتیک مولکولی و مهندسی ژنتیک)، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران. | ||
2استاد گروه بهنژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران. | ||
3استاد گروه بهنژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز- تبریز- ایران | ||
چکیده | ||
تنشهای غیر زنده بهخصوص تنش کمآبی در گیاهان باعث ایجاد تنش اکسایشی و تولید گونههای فعال اکسیژن (ROS) و در نتیجه آسیب جدی به DNA، پروتئین و ساختار درونی گیاهان میشوند. گیاهان بهمنظور مقابله با این تنشها دارای مکانیسمها و سیستمهای دفاع آنزیمی و غیر آنزیمی هستند. هم اکنون باتوجه به تغییرات اقلیمی، تنش کمآبی یکی از مشکلات اصلی تولید در بخش کشاورزی است. باتوجه به اهمیت مطالعات مولکولی و پاسخهای مولکولی و آنزیمی گیاهان تحت شرایط تنشهای غیر زنده، بیان نسبی ژنهای کاتالاز و DREB-2 با تکنیک واکنش زنجیرهای پلیمراز زمان واقعی (Real Time-PCR) و فعالیت آنزیمی کاتالاز در دو ژنوتیپ SC706 و SC260 ذرت تحت تنش کمآبی موردمطالعه قرار گرفته است. آزمایش در شرایط مزرعهای و بهصورت اسپلیتپلات در قالب طرح پایه بلوکهای کامل تصادفی با سه تکرار و در سه شرایط آبیاری نرمال، تنش ملایم و تنش شدید کمآبی اجرا شد. نتایج آنالیز بیان نسبی ژنها اختلاف معنیداری بین تیمارها را نشان داد بهصورتیکه با افزایش شدت تنش کمآبی نسبت به آبیاری نرمال بر میزان فعالیت آنزیمی و بیان نسبی ژنهای مورد بررسی افزوده شد. تحت تنش کمآبی شدید و ملایم نسبت به آبیاری نرمال ژن DREB-2 بهترتیب با 690 و 211 درصد بیشترین درصد افزایش را نشان دادند. در این مطالعه براساس فعالیت آنزیم کاتالاز و میزان بیان ژنهای CAT، DREB-2 ژنوتیپ SC706 نسبت به SC260 از عملکرد بالاتر، رفتار فیزیولوژیکی-مولکولی مناسبتر و بیان بیشتر ژنها برخوردار بودند که نشان دهنده تحمل بیشتر ژنوتیپ SC706 نسبت بهSC260 تحت شرایط تنشهای اعمال شده در این بررسی میباشد که میتوان از این نتایج در برنامههای بهنژادی مولکولی ذرت بهره گرفت. | ||
کلیدواژهها | ||
آنزیم آنتی اکسیدان کاتالاز؛ تنش کم آبی؛ بیان ژن های کاتالاز و DREB-2؛ ذرت | ||
موضوعات | ||
بیوتکنولوژی و تنش های زنده و غیرزنده | ||
عنوان مقاله [English] | ||
Evaluation of Catalase and DREB-2 Gene Expression in Maize (Zea mays L.) Genotypes under Water Deficit Stress Condition | ||
نویسندگان [English] | ||
Khosro Mafakheri1؛ Mostafa Valizadeh2؛ Seyed Abolghasem Mohammadi3 | ||
1Ph.D. of Plant Breeding-Molecular Genetic and Genetic Engineering, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. | ||
2Professor, Plant Breeding and Biotechnology Department, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. | ||
3Professor, Plant Breeding and Biotechnology Department, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. | ||
چکیده [English] | ||
Abiotic stresses, especially water deficit stress in plants, cause oxidative stress and as a result, they produce reactive oxygen species (ROS) and cause serious damage to the DNA, protein and internal structure of plants. Plants have enzymatic and non-enzymatic defense mechanisms and systems to deal with these stresses. Currently due to climate changes, Water deficit stress is one of the problems of agricultural production. Given the importance of molecular studies and molecular and enzymatic responses of plants under abiotic stress conditions, therefore, relative expression of catalase and DREB-2 genes were studied by RT-PCR and catalase enzymatic activity were studied in SC706 and SC260 genotypes. The experiment was performed as a split plot based on a randomized complete block design with three replications and three irrigation conditions of (normal irrigation, intermediate stress and severe water deficit stress) under field condition. Results of relative expression analysis of genes showed a significant difference between the treatments so that by increasing the intensity of water stress compared to normal irrigation, the amount of enzyme activity and relative expression of the studied genes were increased. Under severe and intermediate water deficit stress, compared to normal irrigation, DREB-2 gene showed the highest increase with 690 and 211% respectively. In this study, based on the activity of catalase enzyme and the expression of CAT genes, DREB2 of SC706 genotype had higher performance than SC260, more appropriate physiological-molecular behavior and more expression of genes, which indicates more tolerance of SC706 genotype than SC260. The stress conditions applied in this study are the results that can be used in molecular breeding programs of maize. | ||
کلیدواژهها [English] | ||
Antioxidant enzymes, water deficit stress, Gene expression, maize | ||
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