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شناسایی و بررسی الگوی بیان برخی از ژنهای HSF در عدس (Lens culinaris L.)، تحت تنشکمآبی | ||
فصلنامه علمی زیست فناوری گیاهان زراعی | ||
دوره 11، شماره 37، خرداد 1401، صفحه 17-34 اصل مقاله (742.38 K) | ||
نوع مقاله: علمی پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/cb.2022.63041.1872 | ||
نویسندگان | ||
سمیه ابراهیمی1؛ احمد اسماعیلی* 2؛ سید سجاد سهرابی3؛ حسن ترابی پوده4 | ||
1دانشجوی کارشناسی ارشد، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرمآباد، ایران. | ||
2استاد، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرمآباد، ایران. | ||
3دکتری تخصصی، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرمآباد، ایران. | ||
4دانشیار، گروه مهندسی آب، دانشکده کشاورزی، دانشگاه لرستان، خرمآباد، ایران. | ||
چکیده | ||
تنشهای غیرزیستی از جمله کمآبی در گیاهان منجر به تغییرات فیزیولوژیکی و بیوشیمیایی میشوند. گیاهان مانند سایر موجودات زنده با تنظیم بیان ژن به تغییرات محیطی پاسخ میدهند. عوامل رونویسی، کلیدیترین عناصر مولکولی برای تنظیم بیان ژنها بهشمار میروند. نقش عوامل رونویسی شوک حرارتی (HSFs) در مکانیسم مولکولی پاسخ به انواع تنشهای غیرزیستی مورد تأیید قرارگرفته است، از این رو، در مطالعه حاضر برای شناسایی، طبقهبندی و بررسی تغییرات بیانHSFها در عدس تحت تنش کمآبی از تجزیه و تحلیل دادههای توالییابی شده RNA استفاده شد و در نهایت بیان برخی از رونوشتها با استفاده از روش qRT-PCR مورد بررسی قرار گرفت. از مجموع رونوشتهای سرهمبندی شده عدس، 35 رونوشت متعلق به سه کلاس HSF شناسایی شد. همچنین نتایج نشان داد که در شرایط کمآبی از میان HSFهای شناساییشده، بیان چهار رونوشت شامل HSFA9 و HSFA2 افزایش و در مقابل بیان HSFA6B و HSFB4 کاهشیافته است. علاوه بر این یافتههای ما نشان داد که در پاسخ به تنش خشکی تغییری در بیان رونوشتهای HSF کلاس C دیده نمیشود. بهطورکلی، یافتههای این پژوهش بینشی در مورد ژنهای HSF عدس و نقش احتمالی آنها در پاسخ به تنش کمآبی ایجاد نمودهاست که میتوان از آن بهعنوان پایهای در آزمایشهای آتی برای درک بهتر مکانیسم مولکولی تحمل عدس به تنش کمآبی استفاده نمود. | ||
کلیدواژهها | ||
تنشکمآبی؛ عوامل رونویسی شوک حرارتی؛ عدس؛ qRT-PCR؛ RNA-seq | ||
موضوعات | ||
اصلاح نباتات مولکولی | ||
عنوان مقاله [English] | ||
Identification and expression pattern analysis of some HSF genes in lentil (Lens culinaris L.), under water deficit condition | ||
نویسندگان [English] | ||
Somayeh Ebrahimi1؛ Ahmad Ismaili2؛ Seyed Sajad Sohrabi3؛ Hasan Torabi Podeh4 | ||
1M.Sc. Student, Department of Plant Production and Genetic Engineering, Faculty of Agriculture, Lorestan University, Khorramabad, Iran. | ||
2Professor, Department of Plant Production and Genetic Engineering, Faculty of Agriculture, Lorestan University, Khorramabad, Iran. | ||
3Ph.D., Department of Plant Production and Genetic Engineering, Faculty of Agriculture, Lorestan University, Khorramabad, Iran. | ||
4Associate Professor, Department of Water Engineering, Faculty of Agriculture, Lorestan University, Khorramabad, Iran. | ||
چکیده [English] | ||
Abiotic stresses, including drought in plants, lead to physiological and biochemical changes that are controlled by regulating gene expression. Transcription factors are considered as the most key molecular elements for regulating genes in response to biotic or abiotic stresses. The role of Heat shock transcription factors (HSFs) in the molecular mechanism of response to various abiotic stresses has been confirmed; therefore, this study used the analysis of RNA sequencing data to identify, classify and evaluate changes in HSF expression in lentil under water-deficit stress, and finally, the expression of some transcripts were examined using qRT-PCR. From the total assembled transcripts of lentil, 35 transcripts belonging to three HSF classes were identified. Also, according to the results, the expression of 14.28% of the identified transcripts, which often belonged to class A, is altered in lentil under water-deficit stress. The expression of 14.28% of the identified transcripts, most of which belonged to class A, is altered in lentil under water-deficit stress. In general, the results show that changes in the expression of some transcripts of one HSF gene take precedence over those of other transcripts of that gene in response to drought stress; therefore, it is of particular importance to study alternative splicing in response to this environmental factor in lentil. The HSF genes identified in this study can be used in future experiments to understand better the molecular mechanism of water-deficit stress tolerance in lentil. | ||
کلیدواژهها [English] | ||
Water-deficit, HSFs, Lentil, qRT-PCR, RNA-seq | ||
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