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تاکسونومی ماهی خیاطه (Alburnoides spp.) در ایران | ||
فصلنامه علمی زیست شناسی جانوری تجربی | ||
دوره 11، شماره 2 - شماره پیاپی 42، آذر 1401، صفحه 1-22 اصل مقاله (1.07 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/eab.2023.31495.1530 | ||
نویسندگان | ||
یزدان کیوانی* 1؛ صدیقه محمودی2؛ سالار درافشان1؛ منصوره ملکیان1 | ||
1دانشیار، دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان، اصفهان 8415683111، ایران | ||
2کارشناس ارشد شیلات، دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان، اصفهان، ایران | ||
چکیده | ||
از ایران تا کنون ۱۳ گونه از جنس Alburnoides گزارش شده که در پژوهش حاضر وضعیت آرایهشناختی گونههای A. petrubanarescui، A. namaki، nicolausi .A، A. idignensis،A. eichwaldii و A. qanati با استفاده از صفات ریختسنجی نسبی و شمارشی و ژن سیتوکروم b مورد ارزیابی قرار گرفت. صفات ریختسنجی و شمارشی در ۱۴۴ نمونه بررسی شد. در مطالعات مولکولی سه نمونه از محصول پی.سی.آر هر گونه توالییابی شد. در تجزیه به مؤلفههای اصلی صفات نسبی اندازشی همپوشانی بالایی بین گونهها مشاهده شد، اما تا حدودی A. nicolausi و A. idignensis براساس مؤلفه اول و A. petrubanarescui و A. idignensis براساس مؤلفه دوم از همدیگر تفکیک شدند. در صفات شمارشی نیز به نسبت کمتری همپوشانی دیده شد. گونههای A. nicolausi و A. idignensis براساس مؤلفه اول از یکدیگر مجزا شدند و گونه A. eichwaldii براساس مؤلفه دوم از سایر گونهها جدا شد. در تجزیه خوشهای صفات نسبی اندازشی دو گونه A. namaki و A. nicolausi و همچنین دو گونه A. qanati و A. eichwaldii شباهت بیشتری با یکدیگر داشتند، اما در صفات شمارشی، دو گونه A. qanati و A. eichwaldii دارای شباهت بیشتری با یکدیگر بودند. در تجزیه دادههای مولکولی گونههای A. eichwaldii و A. petrubanarescui بیشترین شباهت (99 درصد) و گونه A. idignensis (85 درصد) بیشترین تفاوت را نسبت به دیگر گونهها نشان دادند. همچنین درختهای فیلوژنی حاصل از روشهای حداکثر احتمال و حداکثر پارسیمونی، گونه A. idignensis را از دیگر گونهها مجزا کرد و دو گونه A. eichwaldii و A. petrubanarescui قرابت بیشتری با یکدیگر داشتند. | ||
کلیدواژهها | ||
تاکسونومی؛ ماهی خیاطه؛ صفات ریختسنجی؛ صفات شمارشی؛ سیتوکروم b | ||
عنوان مقاله [English] | ||
Taxonomy of Spirlins (Alburnoides spp.) in Iran | ||
نویسندگان [English] | ||
Yazdan Keivany1؛ Sedigheh Mahmoodi2؛ Salar Dorafshan1؛ Mansoureh Malekian1 | ||
1Associate Professor, Department of Natural Resources, Isfahan University of Technology, Isfahan 8415683111, Iran | ||
2M. A., Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran | ||
چکیده [English] | ||
So far, 13 species were recently recognized in the Iranian basins. In the present study the variation among A. eichwaldii, A. namaki, A. idignensis, and A. nicolausi, A. qanati A. petrubanarescui has been investigated from morphological and molecular approaches. To study the morphological characteristic, 144 specimens of these six species were used, on which 22 morphometrics were measured. Nine meristics characteristics were counted. Analysis of variance (ANOVA), discriminant analysis, and principal component analysis was performed. In the molecular studies, sequences of the mitochondrial gene, cytochrome b from six species were sequenced and analyzed. The morphological results of the data showed a high similarity among species, but could separate A. nicolausi and A. idignensis according to the PC1. Also A. idignensis and A. petrubanarescui are more similar based on the PC2. Moreover, based on meristic characteristics which were relatively similar, again A. nicolausi and A. idignensis could be separated by the first component. The phylogenetic analysis using Maximum Likelihood (ML) and Maximum-Parsimony (MP) methods supported the monophyletic status of the six species, suggesting their status as different species. The results of the molecular analysis showed that A. eichwaldii and A. petrubanarescui have the most similarity (about 99%) and the A. idignensis has the maximum difference (about 85%) with the other species. These results justify the results of morphometric analysis. | ||
کلیدواژهها [English] | ||
Alburnoides, gene, meristics, mitochondry cytochrome b, morphology, morphometrics, taxonomy | ||
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