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بررسی خاصیت ضدباکتریایی موکوس در گلخورک‌ماهی Boleophthalmus dussumieri (Valenciénnes, 1837) | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 11، شماره 3 - شماره پیاپی 43، اسفند 1401، صفحه 11-20 اصل مقاله (285.13 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2023.65315.1885 | ||
| نویسندگان | ||
| اعظم مکی پور* 1؛ اشرف جزایری2؛ اسماعیل داراب پور3 | ||
| 1کارشناس ارشد، زیست جانوری- بیوسیستماتیک، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید چمران اهواز، اهواز، خوزستان، ایران | ||
| 2استادیار، زیست‌شناسی دریا، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید چمران اهواز، اهواز، خوزستان، ایران | ||
| 3استادیار، میکروبیولوژی، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید چمران اهواز، اهواز، خوزستان، ایران | ||
| چکیده | ||
| گونههای مختلف ماهی بهعلت اختلاف در کیفیت و کمیت پروتئینها و آنزیمهای موجود در موکوس دارای، فعالیت ضدباکتریایی متنوعی هستند. ماهی Boleophthalmus dussumieri یکی از گونههای گلخورک است که طبق مطالعات گذشته از خور موسی گزارش شده است. با توجه به این‌که تاکنون مطالعهای در زمینه فعالیت ضدمیکروبی موکوس این گونه گزارش نشده است، بنابراین هدف این پژوهش بررسی اثر ضدباکتریایی موکوس ماهی B. dussumieri علیه سه سویه باکتری استاندارد (Escherichia coli (ATCC 25922)، Acinetobacter baumannii (ATCC BAA-747)، Bacillus subtillis (ATTC 6633)) و سه سویه باکتری کلینیکی (Escherichia coli، Acinetobacter baumannii، Bacillus anthracis) بود. بعد از تهیه عصاره آبی موکوس، اثر ضدباکتریایی آن در چهار غلظت 25، 50، 100 و 200 میلی گرم بر میلی لیتر به روش انتشار دیسک بررسی شد. دادهها با استفاده از نرمافزار 21 SPSS و آنالیز واریانس یک‌طرفه ANOVA مورد تجزیه و تحلیل قرار گرفتند. نتایج نشان داد که تمامی باکتری‌ها نسبت به عصاره موکوس حساس بودند و هاله عدم رشد بین باکتری‌های مختلف دارای اختلاف معنیدار (05/0P<) بود. هم‌چنین مشخص شد که باکتری‌های گرممثبت با میانگین هاله عدم رشد 12 میلیمتر نسبت به باکتری‌های گرم منفی با میانگین هاله عدم رشد 9 میلیمتر حساسیت بیش‌تری در برابر عصاره موکوس پوست ماهی B. dussumieri نشان دادند. نتیجههای این پژوهش نشان داد که موکوس پوست ماهی Boleophthalmus dussumieri دارای اثر ضدمیکروبی بوده و قابلیت حذف عاملهای بیماریزا در شرایط آزمایشگاهی را دارد. | ||
| کلیدواژه‌ها | ||
| گلخورک؛ خور موسی؛ فعالیت ضدمیکروبی؛ موکوس؛ Boleophthalmus dussumieri. | ||
| عنوان مقاله [English] | ||
| Investigation on the Antibacterial Features of Mucus in Boleophthalmus Dussumieri Mudskeeper | ||
| نویسندگان [English] | ||
| Azam Makipour1؛ Ashraf Jazayeri2؛ Esmaeil Darabpour3 | ||
| 1M.A., Animal biology-Biosystematics, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Khouzestan, Iran | ||
| 2Assistant Professor, Sea Biology, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Khouzestan, Iran | ||
| 3Assistant Professor, Microbiology, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Khouzestan, Iran | ||
| چکیده [English] | ||
| Due to the difference in the quality and quantity of proteins and enzymes in the mucus of different types of fish, therefore they have different antibacterial activity. Boleophthalmus dussumieri fish is one of the mudskeeper’s species that has been reported from Musa Estuary according to previous studies. Therefore, the aim of this research is to investigate the antibacterial effect of B. dussumieri fish mucus against 3 standard bacterial strains (Escherichia coli (ATCC 25922), Acinetobacter baumannii (ATCC BAA-747), Bacillus subtillis (ATTC 6633)) and 3 clinical bacterial strains (Escherichia coli, Acinetobacter baumannii, Bacillus anthracis). After preparing the aqueous extract of mucus, its antibacterial effect was investigated in four concentrations of 25, 50, 100 and 200 mg/ml by disc diffusion method. Data were analyzed using SPSS 21 software and one-way ANOVA. The results showed that all the bacteria were sensitive to the mucus extract and there was a significant difference (P<0.05) in the growth inhibition zone between different bacteria. It was also found that Gram-positive bacteria with an average non-growth zone of 12 mm showed more sensitivity to B. dussumieri fish skin mucus extract than Gram-negative bacteria with an average non-growth zone of 9 mm. The results of this research showed that the skin mucus of Boleophthalmus dussumieri fish has antimicrobial effect and has the ability to remove pathogenic agents in laboratory conditions. | ||
| کلیدواژه‌ها [English] | ||
| Antimicrobial activity, Boleophthalmus dussumieri, Mudskeeper, Mucus, Musa Estuary | ||
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