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بررسی ساختار و قرابت پروتئینهای دهیدرین توسط ابزارهای بیوانفورماتیک در گیاهان تکلپه و دولپه | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| دوره 13، شماره 2 - شماره پیاپی 46، تیر 1403، صفحه 59-73 اصل مقاله (2.73 M) | ||
| نوع مقاله: علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/cb.2024.70852.1962 | ||
| نویسندگان | ||
| آرمین ساعد موچشی1؛ علی شیرخانی* 2 | ||
| 1بخش تحقیقات زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمانشاه، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرمانشاه | ||
| 2بخش تحقیقات زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمانشاه، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرمانشاه، | ||
| چکیده | ||
| پروتئینهای گروه دهیدرین (Dehydrin: DHN)، گروهی از پروتئینهای مهم دخیل در پاسخ به تنشهای غیر زیستی مانند سرما و خشکی در گیاهان هستند. این پروتئینها به گروهی از پروتئینهای محافظتکننده از سایر پروتئینها به نام type II Late embryogenesis abundant تعلق دارند. با توجه به اهمیت پروتئینهای گروه دهیدرین در گیاهان، در این تحقیق روابط تکاملی این گروه در گیاهان مختلف موردبررسی قرار گرفت. بدین منظور توالیهای پروتئینهای دهیدرین گیاهان مختلف از سایت NCBI استخراج و همردیف گردید. نتایج وجود نواحی حفاظتشده ازجمله موتیف K و S که به ترتیب در واکنش با دیگر پروتئینهای تحت تنش و محافظت از آنها و همچنین انتقال پروتئینهای گروه دهیدرین از سیتوپلاسم به هسته نقش دارند را در بین ژنهای مورد بررسی نشان داد. درخت فیلوژنی بر پایه نواحی حفاظتشده و با روش Neighbor Joining رسم گردید و توالی خطی و درصد اسیدآمینههای موجود در ساختار این پروتئینها بههمراه توالی مکمل ژنومی آنها نیز مورد بررسی قرار گرفت. نتایج نشان داد که پروتئینهای گروه دهیدرین دولپهای و تکلپهایها به دو گروه مجزا تفکیکشده و در هر گروه نیز بر اساس نزدیکی و دوری جنسهای مختلف گیاهی در کلاسترهای مجزا قرار گرفتند. همچنین گیاهان تک و دولپه ازلحاظ توالی خطی اسیدآمینه و درصد آنها هم اختلاف بالایی را نشان دادند. از طرفی، بررسی توالی ژنومی این گیاهان نشاندهنده وجود ساختارهای حفاظتشده و مشابه بود. | ||
| کلیدواژهها | ||
| نش غیر زیستی؛ موتیفهای S و K؛ پروتئینهای Late embryogenesis abundant؛ Neighbor Joining | ||
| موضوعات | ||
| بیوانفورماتیک | ||
| عنوان مقاله [English] | ||
| Structure and similarity of dehydrin proteins using bioinformatics tools in monocotyledons and dicotyledons plants | ||
| نویسندگان [English] | ||
| Armin Saed-Moucheshi1؛ Ali Shirkhani2 | ||
| 1Crop and Horticulture Reseach Department, Kermanshah Agricultural and Natural Resources Research and Education Center (AREEO), Kermanshah, Iran. | ||
| 2Crop and Horticulture Reseach Department, Kermanshah Agricultural and Natural Resources Research and Education Center (AREEO), Kermanshah, Iran. | ||
| چکیده [English] | ||
| Dehydrin (DHN) proteins are a group of proteins that effectively respond to abiotic stresses such as cold and drought stress in plants. These proteins are a subset of protective greater group of proteins called type II Late embryogenesis abundant which are protecting other proteins from stresses shock. Due to the significant effect of dehydrin proteins in plants, specialy under abiotic stresses, the aims of this study were to survey the linear structure along with phylogeny relationship of this proteins’ group in different plants species. The protein linear sequences of different plant species were downloaded from NCBI site and then were aligned using MegaX software. The results of aligning showed highly conserved segments within the considered sequences such as K- and S-segments that are respectively responsible for covering other proteins and protecting them from damaging effects of stresses and transporting dehydrin proteins from cytoplasm to nucleus. Using the sequences’ alignment, phylogenic tree was extracted using Neighbor joining method. Furthermore, linear sequence order of amino acids and their ratio in the structure of these protein were evaluated. Folowing that, the composition of these proteins genomic sequences were considered to compare with the results of amino acids evaluation. The results indicated that dicotyledon and monocotyledon plants can be clearly separated into two distinguished classes based on the amino acid structure of DHN proteins. Similarly, The ratio and order of DNHs linear sequences were distinctly altered between mono- and di-cotyledon plants. Evaluation of genomic base pairs of these proteins showed that there are numerous unchanged motifs with eighter no or a little difference shared among the genomic sequences of DHN proteins. | ||
| کلیدواژهها [English] | ||
| Abiotic stress, K and S motifs, Late embryogenesis abundant proteins, Neighbor Joining | ||
| مراجع | ||
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