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تغییرات آسیب‌شناسی بافت آبشش و کبد سیاه ماهی (Capoeta capoeta gracilis) در مواجهه با سم بوتاکلر | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| مقاله 7، دوره 7، شماره 4 - شماره پیاپی 28، اردیبهشت 1398، صفحه 69-81 اصل مقاله (804.22 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2019.5682 | ||
| نویسندگان | ||
| محمد فروهر واجارگاه* 1؛ حامد غفاری فارسانی2؛ سعید شهبازی ناصرآباد3؛ سید علی اکبر هدایتی4 | ||
| 1دانشجوی دکتری بوم شناسی آبزیان، دانشکده منابع طبیعی، دانشگاه گیلان، ایران | ||
| 2دانشجوی دکتری شیلات، باشگاه پژوهشگران جوان و نخبگان، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران | ||
| 3دانشجوی دکتری شیلات ، باشگاه پژوهشگران جوان و نخبگان، واحد یاسوج، دانشگاه آزاد اسلامی، یاسوج، ایران | ||
| 4دانشیار، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
| چکیده | ||
| چکیده موجودات مختلف از جمله آبزیان همواره در معرض آلودگی با سموم کشاورزی هستند، این مطالعه به ارزیابی کلی تغییرات پاتوبیولوژیکی آبشش و کبد سیاه ماهی در مواجهه با غلظتهای تحت کشنده سم بوتاکلر پرداخته است. در ابتدا غلظت کشنده 96 ساعته این سم 46/2 میلیگرم بر لیتر تعیین شد. سپس 96 قطعه ماهی به چهار گروه شامل یک گروه شاهد و سه گروه تجربی (با غلظتهای تحت کشنده 2/0، 4/0 و 8/0 میلیگرم بر لیتر) تقسیم گردیدند و بهمدتدو هفتهدر معرض این غلظتها قرار گرفتند. در پایان دوره آزمایش، مقاطع بافتی تهیه و به روش هماتوکسیلین- ائوزین رنگآمیزی و تغییرات بافتی با میکروسکوپ نوری مطالعه شد. غلظت کشنده سم بوتاکلر برای سیاه ماهی 46/2 میلیگرم بر لیتر بهدست آمد که نشاندهنده سمیت نسبی آن برای این ماهی میباشد، تغییرات شدید بافتشناسی در آبشش شامل هیپرپلازی، کوتاه شدگی لاملای ثانویه، ادم، فیوژن، هموراژ و آتروفی نسبت به گروه شاهد دیده شد. همچنین آسیبهای بافت کبد توسط بوتاکلر موجب بروز انسداد، گرانول تیره، نکروز، صفرا و سینوزوئید در سیاه ماهی شد. نتایج پژوهش حاضر حاکی از آن بود که، با افزایش غلظت سم، آسیبهای بافتی شدیدتری در اندامهای مورد مطالعه مشاهده شد، بنابراین دو بافت آبشش و کبد سیاه ماهی می‌توانند به‌‌عنوان یک بیومارکر مناسب جهت سنجش آلودگی به‌کار روند. | ||
| کلیدواژه‌ها | ||
| واژه‌های کلیدی: هیستوپاتولوژی؛ علف‌کش؛ بوتاکلر؛ سیاه ماهی؛ بافت | ||
| عنوان مقاله [English] | ||
| Histopathological lesions in liver and gill of Capoeta capoeta gracilis in exposed to butachlor | ||
| نویسندگان [English] | ||
| Mohammad Forouhar Vajargah1؛ Hamed Ghafari Farsani2؛ Saeid Shahbazi Naserabad3؛ Aliakbar Hedayati4 | ||
| 1Ph.D. Candidate in Aquatic Ecology, Faculty of Natural Resources, Guilan University, Iran | ||
| 2Ph.D. Candidate in Fisheries, Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran | ||
| 3Ph.D. Candidate in Aquatic Ecology, Young Researchers and Elite Club, Yasooj Branch, Islamic Azad University, Yasooj, Iran | ||
| 4Associate Professor, Faculty of Fisheries and Environmental Sciences, Gorgon University of Agricultural Sciences and Natural Resources, Gorgon, Iran | ||
| چکیده [English] | ||
| Abstract Different organisms like aquatics are constantly exposed to pesticide residues, this study's overall assessment patho-biological changes in gills and livers of Capoeta capoeta gracilis during exposure to butachlor pollutant. First of all the 96-hour lethal concentration of the toxin was calculated as 46.2 mg per liter. Then, 96 fish were divided into four groups: one control group and three experimental groups (with concentrations of 0.2, 0.4, and 0.8 mg l-1) and were exposed for 2 weeks. In the end point, histological changes were studied by hematoxylin and eosin staining method in light microscopy. Lethal concentration of this toxin was 2.46 mg/l that means moderate toxicity of this species. There was many histological changes in the gills of fish treated with butachlor include: hyperplasia, shortening the secondary lamella, edema, fusion, hemorrhage and atrophy in compared to the control group. Treatment with liver butachlor cause obstruction, dark granules, necrosis, and bile stagnation and sinusoid dilution in studied fish. The results of this study showed that in both studied organs with increase of toxin concentration, more severe tissue damage was observed, so gill and liver tissues of this fish could be used as an appropriate biomarker for pollutant measurement. | ||
| کلیدواژه‌ها [English] | ||
| Keywords: Butachlor, Capoeta capoeta gracilis, Herbicide, Histopathology, Tissue | ||
| مراجع | ||
|
Abdoli, A.; (2000). The Inland Water Fishes of Iran. Iranian Museum of Nature and Wildlife, Tehran, 378p.
Ahmadivand, S.; Farahmand, H.; Mirvaghefi, A. R.; Eagderi, S.; Shokrpoor, S.; Rahmati-Holasoo, H.; (2014). Histopathological and haematological response of male rainbow trout (Oncorhynchus mykiss) subjected to butachlor. Veterinarni Medicina; 59(9): 433-439.
Alazemi, B.M.; Lewis, J.W.; Andrews, E.B.; (1996(. Gill damage in the freshwater fish Gnathonemus petersii (family: Mormyridae) exposed to selected pollutants: an ultrastructural study. Environment Technology; 17(3): 225-238.
Ateeq, B.; Farah, M.A.; Ali, M.N.; Ahmed, W.; (2002). Clastogenicity of pentachlorophenol, 2,4-D and butachlor evaluated by Allium root tip test. Mutation Research; 514: 105-113.
Belicheva, L. A.; Sharova, J. N.; (2015). Assessment of fish health status under long-term water pollution: Vygozero reservoir, north-west Russia. In Environment. Technology. Resources. Proceedings of the International Scientific and Practical Conference; 2: 368-373.
Boran, H.; Capkin, E.; Altinok, I.; Terzi, E.; (2012): Assessment of acute toxicity and histopathology of the fungicide captan in rainbow trout. Experimental and Toxicological Pathology; 64: 175-179.
Butchiram, M.S.; Tilak, K.S.; Raju, P.W.; (2009). Studies on histopathological changes in the gill, liver and kidney of Channa punctatus (Bloch) exposed to Alachlor. Environment Biology; 30(2): 303-306.
Camargo, M. M.; Martinez, C. B.; (2007). Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotropical Ichthyology; 5(3): 327-336.
Cengiz, E.I.; (2006). Gill and kidney histopathology in the freshwater fish Cyprinus carpio after acute exposure to deltamethrin. Environmental Toxicology and Pharmacology; 22(2): 200-204.
Cengiz, E.I.; Unlu, E.; (2006). Sublethal effects of commercial deltamethrin on the structure of the gill, liver and gut tissues of mosquitofish Gambusia affinis, A microscopic study. Environmental Toxicology and Pharmacology; 21(3): 246-253.
Chen, J.; Dong, X.; Xin, Y.; Zhao, M.; (2011). Effects of titanium dioxide nano-particles on growth and some histological parameters of zebrafish (Danio rerio) after a long-term exposure. Aquatic Toxicology; 101(3-4): 493-499.
Das, B.K.; Mukherjee, S.C.; (2003). Toxicity of cypermethrin in Labeo rohita fingerlings: biochemical, enzymatic and haematological consequences. Comparative Biochemistry and Physiology Part C: Toxicology Pharmacology; 134(1): 109-121.
Dezfuli, B. S.; Simoni, E.; Giari, L.; Manera, M.; (2006). Effects of experimental terbuthylazine exposure on the cells of Dicentrarchus labrax (L.). Chemosphere; 64(10): 1684-1694.
Fanta, E.; Rios, F.S.A.; Romão, S.; Vianna, A.C.C.; Freiberger, S.; (2003). Histopathology of the fish Corydoras paleatus contaminated with sublethal levels of organophosphorus in water and food. Ecotoxicology and environmental safety; 54(2): 119-130.
Farsani, H.G.; Rashidian, G.; Narimanizad, F.; Khodadadi, M.; Gerami, M. H.; (2015). Histopathology and Biochemical Analysis of Common Carp (Cyprinus carpio) Exposed to Sublethal Concentrations of Carboxin-thiram (Vitavax thiram). Journal of Fisheries and Aquatic Science; 10(5): 337-346.
Finney, D.; )1971(. Probit analysis, a statistical treatment of the sigmoid response curve. Cambridge. 256 p.
Forouhar Vajargah, M.; Mohamadi Yalsuyi, A.; Hedayati, A.; Faggio, C.; (2018). Histopathological lesions and toxicity in common carp (Cyprinus carpio L. 1758) induced by copper nanoparticles. Microscopy research and technique; 81(7): 724-729.
Gholamian, S.; (2014). The effect of Butachlor sublethal concentrations on some biochemical indices and liver tissue of Persian sturgeon (Acipenser persicus). Journal of Marine Biology; 6(2): 1-9.
Hedayati, A.; Jahanbakhshi, A.; Ghaderiremazi, F.; (2013). Aquatic toxicology. Publications University of Agricultural Sciences and Natural Resources, Gorgan, 210p. (In Persian)
Hedayati, A.; Vajargah, M.F.; Yalsuyi, A.M.; Abarghoei, S.; Hajiahmadyan, M.; (2014). Acute toxicity test of pesticide abamectin on common carp (Cyprinus carpio). Journal of Coastal Life Medicine; 2(11): 841-844.
Jayachandran, K.; Pugazhendy, K.; (2009). Histopathological changes in the gill of Labeo rohita (Hamilton) fingerlings exposed to atrazine. American-Eurasian Journal of Scientific Research; 4(3): 219-221.
Keizer; J.D.; Agostino; G.; Vittozzi; L.; (1990). Metabolism baded differences of diazinon toxicity to guppy (P.reticulata) and zebra fish (Brachydanio rario). 12th ESCPB Conference.
Khabbazi, M.; Harsij, M.; Hedayati, S.A.A.; Gerami, M.H.; Ghafari-Farsani, H.; (2014 a). Histopathology of rainbow trout gills after exposure to copper. Iranian Journal of Ichthyology; 1(3): 191-196.
Khabbazi, M.; Harsij, M.; Hedayati, S.A.A.; Gerami, M.H.; Ghafari-Farsani, H.; (2014 b). Investigation on destructive effect of water-born CuO nanoparticles on gill histopathology of rainbow trout, Oncorhynchus mykiss. Aquatic Physiology and Biotechnology; 2(1): 75-88.
Lasheidani, M.F.; Balouchi, S.N.; Keyvan, A.; Jamili, S.; Falakrou, K.; (2008). Effects of butachlor on density, volume and number of abnormal sperms in caspian kutum (Rutilus frisii kutum, Kamenskii 1901). Research Journal of Environmental Sciences;, 2(6): 474-482.
Laurèn, D.J.; McDonald, D.G.; (1985). Effects of copper on branchial ionoregulation in the rainbow trout, Salmo gairdneri Richardson. Journal of Comparative Physiology, 155(5): 635-644.
Martoja, R.; Martoja-Pierson, M.; (1967). Initiation Aux Techniques de l histology animale. Masson et Cie, Paris, 345 p.
Maxwell, L. B.; Dutta, H. M.; (2005). Diazinon- induced endocrine disruption in blue gill sunfish, lepomis macrochirus. Ecotoxicology and Environmental Safety; 60(1): 21-70.
OECD (Organisation for Economic Co-operation and Development).; )1993(. OECD Guidelines for Testing of Chemicals OECD, Organization for Economic. Paris
Ogundiran, M.A.; Fawole, O.O.; Adewoye, S.O.; Ayandiran, T.A.; (2010). Toxicological impact of detergent effluent on juvenile of African Catfish (Clarias gariepinus) (Buchell 1822). Agriculture and Biology Journal of North America; 1(3): 330-342.
Pandey, S.; Kumar, R.; Sharma, S.; Nagpure, N.S.; Srivastava, S.K.; Verma, M.S.; (2005). Acute toxicity bioassays of mercuric chloride and Malathion on air-breathing fish Channa punctatus (Bloch). Ecotoxicology and environmental safety; 61(1): 114-120.
Ramah, K.; (2013). Histopathological study on the effect of rice herbicides on grass carp (Ctenopharyngodan idella). African Journal of Biotechnology; 10(7): 1112-1116.
Rodrigues, R.V.; Miranda-Filho, K.C.; Gusmão, E.P.; Moreira, C.B.; Romano, L.A.; Sampaio, L.A.; (2010). Deleterious effects of water-soluble fraction of petroleum, diesel and gasoline on marine pejerrey Odontesthes argentinensis larvae. Science of the Total Environment; 408(9): 2054-2059.
Roy, N.K.; (2002). Chemestery of pesticides. Indian Agriculture. Research. 346 pp.
Sadeghi, A.; Hedayati, A.; (2014). Investigation of Acute Toxicity Diazinon, Deltamethrin, Butachlor and pretilachlor on Zebra Cichlid (Cryptoheros nigrofasciatus). Iranian Journal of Toxicology; 8(25): 1086-1092.
Sadeghi, A.; Imanpoor, M.R.; Hedayati, A.; Masoodi, E.; (2013). Determination of LC, NOEC and LOEC for Toxin Butachlor on Gambusia. World Journal of Zoology; 8(3): 308-311.
Safavi, F.; Poorbagher, H.; Javanshir, A.; Eagderi, S.; (2015). Effect of different concentration of cypermethrin on gill of Capoeta damascina (Valenciennes, 1842). Journal of Aquatic Ecology; 4(4): 88-95.
Schwaiger, J.; Fent, K.; Stecher, H.; Ferling, H.; Negele, R.D.; (1996). Effects of sublethal concentrations of triphenyltinacetate on rainbow trout (Oncorhynchus mykiss). Archives of Environmental Contamination and Toxicology; 30(3): 327-334.
Smith, C. J.; Shaw, B. J.; Handy, R. D.; (2007). Toxicity of single walled carbon nanotubes to rainbow trout, (Oncorhynchus mykiss): respiratory toxicity, organ pathologies, and other physiological effects. Aquatic toxicology; 82(2): 94-109.
Tilak, K. S.; Veeraiah, K.; Thathaji, P. B.; (2005). Histopathological changes in gill tissues of the fish Channa punctatus exposed to sublethal concentration of butachlor and machete. Aquatic Biology; 20(1): 111-115.
Tilak, K. S.; Veeraiah, K.; Thathaji, P. B.; (2007). Histopathological changes in the kidney of the fish Channa punctata exposed to sublethal concentration of Butachlor and Machete. Journal of Ecotoxicology and Environmental Monitoring; 17(2): 129.
Tomlin, C.; (1994). The Pesticide Manual. 10th ed. Crop Protection Publications, British Crop Protection Council, Farnham, Surrey, UK. 1341 pp.
Turan, C.; (2008). Molecular systematics of the Capoeta (Cypriniformes: Cyprinidae) species complex inferred from mitochondrial 16s rDNA sequence data. Acta Zoological; 51: 1-14.
Vajargah, M. F.; Hedayati, A.; (2017). Acute Toxicity of Butachlor to Rutilus rutilus caspicus and Sander lucioperca in Vivo Condition. Transylvanian Review of Systematical and Ecological Research; 19(3): 85-92.
Vajargah, M.F.; Hedayati, A.; Yalsuyi, A.M.; Abarghoei, S.; Gerami, M.H.; Farsani, H.G.; (2014). Acute toxicity of Butachlor to Caspian Kutum (Rutilus frisii Kutum Kamensky, 1991). Journal of Environmental Treatment Techniques; 2(4): 155-157.
Van, H.D.; Vosloo, A.; Nikinmaa, M.; (2004). Effects of short-term copper exposure on gill structure, methallothionein and hypoxia-inducible factor- 1á (HIF-1á) levels in rainbow trout (Oncorhynchus mykiss). Aquatic Toxicology; 69: 271-280.
Velisek, J.; Svobodova, Z.; Piackova, V.; (2009). Effects of acute exposure to bifenthrin on some haematological, biochemical and histopathological parameters of rainbow trout (Oncorhynchus mykiss). Veterinarni Medicina; 54(3): 131-137.
Velmurugan, B.; Mathews, T.; Cengiz, E.I.; (2009). Histopathological effects of cypermethrin on gill, liver and kidney of fresh water fish Clarias gariepinus (Burchell, 1822), and recovery after exposure. Environmental technology; 30(13): 1453-1460.
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